cd14  (R&D Systems)

Journal: bioRxiv

Article Title: IFI16/AIM2 inflammasomes control Gal-9 and PVR in myeloid cells from PWH and their targeting improves immunotherapy against HIV-1

doi: 10.64898/2026.01.26.701473

Figure Lengend Snippet: (A): ELISA analysis of plasma concentration of IL-1β, comparing PWH with normal CD4/CD8 T cell ratio (defined as ≥1) and low CD4/CD8 T cell ratio (defined as < 0.8). Statistical significance was calculated using two-tailed Wilcoxon test. (B): Spearman correlation between plasma concentrations of soluble CD14 (ssCD14) and IL-1β. P and R values are shown (C): Expression of Gal-9 (Gal9; higher plot) or PVRhi (lower plot) in activated CD40hi in Mo from HIV- 1-negative controls, and PWH with low CD4/CD8 T cell ratio or normal CD4/CD8 T cell ratio after 16-hour stimulation with PAMPs mimicking bacterial translocation (LPS, red; Flagellin, Flag, purple) or viral replication (CL097, ssRNA, orange; Poly I:C, dsRNA, blue). Statistical significance was calculated using Kruskal-Wallis test for comparison of stimulation with the same PAMP between groups (*p<0.05; **p<0.01), and One-Way ANOVA Friedman test with Dunn’s multiple comparison test for comparison within the same group (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001).

Article Snippet: Plasma levels of soluble CD14, IL-6 and IL-1β were determined using the Human CD14 DuoSet ELISA, the Human IL-6 DuoSet ELISA kits (both from R&D systems) or the Quantikine ELISA Human IL- 1β/IL-1F2 kit (R&D systems), respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Concentration Assay, Two Tailed Test, Expressing, Translocation Assay, Comparison

Journal: bioRxiv

Article Title: IFI16/AIM2 inflammasomes control Gal-9 and PVR in myeloid cells from PWH and their targeting improves immunotherapy against HIV-1

doi: 10.64898/2026.01.26.701473

Figure Lengend Snippet: (A-B): Histological analysis of IFI-16 (A) or AIM2 (B) (green) and Caspase-1 (red) expression and DAPI (blue) in the spleen from a viremic HIV-1 infected humanized BLT mouse. Quantifications of cells positive cells for each inflammasome sensor alone (top plots), co-expressing Caspase 1 (CASP1) (middle plots) or both Caspase 1 and CD14 (bottom plots) are shown on the right. (C): Histological analysis of Gal-9 (Gal9; green), CD14 (white) and Caspase-1 (Casp1, red) expression and DAPI (blue) in the spleen from a viremic HIV-1 infected humanized BLT mouse. Zoomed area demonstrating co-expression between Gal9, CD14 and Caspase-1 are shown on the right. Arrows highlight cells co-expressing the mentioned markers

Article Snippet: Plasma levels of soluble CD14, IL-6 and IL-1β were determined using the Human CD14 DuoSet ELISA, the Human IL-6 DuoSet ELISA kits (both from R&D systems) or the Quantikine ELISA Human IL- 1β/IL-1F2 kit (R&D systems), respectively.

Techniques: Expressing, Infection

Journal: bioRxiv

Article Title: IFI16/AIM2 inflammasomes control Gal-9 and PVR in myeloid cells from PWH and their targeting improves immunotherapy against HIV-1

doi: 10.64898/2026.01.26.701473

Figure Lengend Snippet: (A): ELISA analysis of plasma concentration of IL-1β, comparing PWH with normal CD4/CD8 T cell ratio (defined as ≥1) and low CD4/CD8 T cell ratio (defined as < 0.8). Statistical significance was calculated using two-tailed Wilcoxon test. (B): Spearman correlation between plasma concentrations of soluble CD14 (ssCD14) and IL-1β. P and R values are shown (C): Expression of Gal-9 (Gal9; higher plot) or PVRhi (lower plot) in activated CD40hi in Mo from HIV- 1-negative controls, and PWH with low CD4/CD8 T cell ratio or normal CD4/CD8 T cell ratio after 16-hour stimulation with PAMPs mimicking bacterial translocation (LPS, red; Flagellin, Flag, purple) or viral replication (CL097, ssRNA, orange; Poly I:C, dsRNA, blue). Statistical significance was calculated using Kruskal-Wallis test for comparison of stimulation with the same PAMP between groups (*p<0.05; **p<0.01), and One-Way ANOVA Friedman test with Dunn’s multiple comparison test for comparison within the same group (*p<0.05; **p<0.01; ***p<0.001; ****p<0.0001).

Article Snippet: Plasma levels of soluble CD14, IL-6 and IL-1β were determined using the Human CD14 DuoSet ELISA, the Human IL-6 DuoSet ELISA kits (both from R&D systems) or the Quantikine ELISA Human IL- 1β/IL-1F2 kit (R&D systems), respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Concentration Assay, Two Tailed Test, Expressing, Translocation Assay, Comparison

Journal: bioRxiv

Article Title: IFI16/AIM2 inflammasomes control Gal-9 and PVR in myeloid cells from PWH and their targeting improves immunotherapy against HIV-1

doi: 10.64898/2026.01.26.701473

Figure Lengend Snippet: (A-B): Histological analysis of IFI-16 (A) or AIM2 (B) (green) and Caspase-1 (red) expression and DAPI (blue) in the spleen from a viremic HIV-1 infected humanized BLT mouse. Quantifications of cells positive cells for each inflammasome sensor alone (top plots), co-expressing Caspase 1 (CASP1) (middle plots) or both Caspase 1 and CD14 (bottom plots) are shown on the right. (C): Histological analysis of Gal-9 (Gal9; green), CD14 (white) and Caspase-1 (Casp1, red) expression and DAPI (blue) in the spleen from a viremic HIV-1 infected humanized BLT mouse. Zoomed area demonstrating co-expression between Gal9, CD14 and Caspase-1 are shown on the right. Arrows highlight cells co-expressing the mentioned markers

Article Snippet: Plasma levels of soluble CD14, IL-6 and IL-1β were determined using the Human CD14 DuoSet ELISA, the Human IL-6 DuoSet ELISA kits (both from R&D systems) or the Quantikine ELISA Human IL- 1β/IL-1F2 kit (R&D systems), respectively.

Techniques: Expressing, Infection